| 摘要: |
| 为寻找新型α-葡萄糖苷酶抑制(αglucosidase inhibition,α-GD)活性化合物来源，针对前期研究发现的具有α-GI活性的海绵附生微生物坚强芽孢杆菌(Bacllus firmus),对其发酵培养条件进行优化，并对其活性组分中主要成分进行初步分析.以α-GI活性为主要检测对象，选出蛋白胨、酵母膏、NaCl和pH值共4个因素进行正交优化试验.结合菌株生长情况和代谢产物产量指标，发现在MB培养基的基础上，蛋白胨质量浓度3g/dm³、酵母膏质量浓度3g/dm³、NaCl质量浓度19.5g/dm³、pH值为7.5的组合为最优培养条件.在优化后的培养条件下，规模化发酵该菌株，提取其代谢产物粗提物，经活性追踪下的二次色谱柱分离，获得一个高抑制活性组分，抑制率达82.4%.该组分经GC-MS分析后发现，活性组分中主要成分为环二肽类及哌啶酮类物质.本研究结果为寻找新型α-GI活性化合物提供了一个新的、可扩大生产的来源. |
| 关键词: 海洋生物学 海绵附生菌 坚强芽孢杆菌 代谢产物 α-葡萄糖苷酶抑制活性 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(41076097,41106113,41271521):教育部科学技术研究重点专项资助项目(211065):江苏省教育厅高校自然科学基金资助项目(11KJB170011) |
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| Optimization on culture conditions for a sponge-associated bacteriumBacillus firmus and preliminary analysis on its productswith inhibiting activity against α-glucosidase |
| QIU Jingjing,ZHA Xinyao,JIANG Wei,JIN Cuili,ZHOU Xiaojian |
| (College of Envirommental Science &Engineering,Yangzhou University,Yangzhou 225127,China;Marine Science &Technology Institute,Yangzhou University,Yangzhou 225127,China) |
| Abstract: |
| In onder to find new and active α-glucosidase inhibitors,the medium composition of a sponge-associatedbacterium Bacillus fimus,which had been found possessing α-glucosidase inhibition(α-GI)activity in the previ-ous studies,was optimized through the orthogonal test.The active components in crude extracts of this strain werealso isolated and analyzed.For an effective αglucosidase inhibition,rapid cell growth(in terms of cell wet weight)and high crude extract production of the metabolites,an orthogonal test was conducted and it indicates that the com-bination of peptone 3g/dm³,yeast extract 3g/dm³,NaCl 19.5g/dm³and pH=7.5 is the optimal conditionsbased on the MB medium.The strain was fermented in large scale under the optimized conditions and the metabo lites cude extract was extracted.After twice separation guided by αGI activities on silicagel column,a sub frac-tion with high activity of 82.4%against αglucosidase was then obtained.The GC-MS analysis indicates that cyclicdipeptides and piperidones contributed major components in this active sub-fraction.Present study provided a newand applicable resource for the promising αglucosidase inhibitors. |
| Key words: maine biology sponge-associated bacterium Bacillus fimus metabolites α-glucosidase inhibitionactivity |
