| 摘要: |
| 本研究开展了黄鳍东方鲀(Fugu xanthopterus)和双斑东方鲀(Fugu bimaculatus)人工正反杂交实验，通过ISSR分子标记技术进行遗传分析，结果表明：亲本及其正反交子一代个体间都有其特异的ISSR分子标记，亲本群体的遗传多样性指数(H)分别为0.3148和0.2765,正反交子代群体的遗传多样性指数分别为0.3362和0.3257,黄鳍东方鲀和双斑东方鲀群体间的遗传距离(D)较远为0.895,反交群体与双斑东方鲀群体间的遗传距离最近为0.169.实验数据表明双斑东方鲀对杂交子代的影响更为显著. |
| 关键词: 海洋生物学 黄鳍东方鲀 双斑东方鲀 杂交子代 ISSR |
| DOI： |
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| 基金项目:福建省海洋与渔业厅资助项目(闽海渔科0415号) |
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| Genetic analysis with ISSR method on reciprocal hybrid progenybetween Fugu xanthopterus and Fugu bimaculatus |
| ZHONG Jianxing,LIU Bo,ZHONG Ran,YANG Shengchang |
| (Fishery Research Institute of Fujan,Xiamen 361013,China;College of LifeSciences,Xiamen University,Xiamen 361005,China) |
| Abstract: |
| In this study artificial cnssbreeding between Fugu xanthopteru and Fugu bimaculatus was made.The In-ter-Simple Sequence Repeats(ISSR)technology was applied to analyze the genetic diversity between Fugu xan-thopterus,Fugu bimaculatus and their hybrid progeny(F1).The results showed that there were some particular IS-SR loci among Fugu xanthopterus,Fugu bimaculatus and their reciprocal hybrids.The genetic diversity index(H)of parent was0.3148 and 0.2765 while between their reciprocal hybrids was 0.3362 and 0.3257.The geneticdistance(D)between Fugu xanthopterus and Fugu bimaculatus was 0.895 while the genetic distance between thereversed hybrid population and Fugu bimaculatus was 0.169.This experiment indicated that the effect of Fugu bi-maculatus on hybrid progeny was much more significant than Fugu xanthopterus. |
| Key words: marine biology Fugu xanthopterus Fugu bimaculatus hybrid progeny ISSR |
