| 摘要: |
| Fc受体（Fc Receptors，FcRs）是一类与免疫球蛋白的Fc段特异性结合，介导多种免疫反应的受体分子。本研究克隆得到了大黄鱼（Larimichthys crocea）6个FcRs基因，分别是LcFcγRⅠL、LcFcγRⅡ、LcFcγRⅡaL、LcFcγRⅢ、LcFcRL5和LcFcRLA，其开放阅读框（ORF）大小分别为1 070、2 486、1 145、 1 955、4 125、1 659 bp。大黄鱼的6个LcFcRs都具有一个信号肽和2~8个免疫球蛋白（Ig）结构域，LcFcγRⅡ、LcFcγRⅢ和LcFcRL5还包含一个跨膜区。实时荧光定量PCR结果显示LcFcγRⅠL、LcFcγRⅡ、LcFcγRⅡaL和LcFcγRⅢ主要在鳃或肠等黏膜组织中表达，而LcFcRL5和LcFcRLA主要在脾和头肾等系统免疫组织中表达；革兰氏阴性细菌细胞壁脂多糖（LPS）刺激后，这6个LcFcRs基因头肾和脾脏中的表达在不同时间出现了显著上调；双链RNA病毒类似物Poly(I:C)刺激后，6个LcFcRs在头肾和脾脏中的表达出现不同程度的上调，而LcFcRL5在脾脏中的表达显著上调，在头肾中则明显下调，提示大黄鱼FcRs可能在LPS和Poly(I:C)诱导的免疫反应中起着重要但又不同的作用。 |
| 关键词: 海洋生物学 大黄鱼 FcRs 免疫球蛋白 分子克隆 |
| DOI：10.3969/J.ISSN.2095-4972.20220725002 |
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| 基金项目:福州海洋研究院基金（2021F02）；国家自然科学基金（31802333） |
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| Cloning of large yellow croaker （Larimichthys crocea） FcRs gene and preliminary functional analysis in immune response |
| YANG Yusheng,CHENG Anyi,ZHANG Yameng,CUI Zhengwei,CHEN Xinhua |
| (Institute of Oceanology， Fujian Agriculture and Forestry University, Key Laboratory of Marine Biotechnology of Fujian Province， Fuzhou 350002，China) |
| Abstract: |
| Fc receptors (FcRs) are a class of receptor that specifically binds to the Fc segment of immunoglobulin and mediates a variety of immune responses. In this study, six FcR genes were cloned from large yellow croaker (Larimichthys crocea), including LcFcγRⅠL, LcFcγRⅡ, LcFcγRⅡaL, LcFcγRⅢ, LcFcRL5 and LcFcRLA, and their open reading frame is 1 070, 2 486, 1 145, 1 955, 4 125 and 1 659 base pair, respectively. Each of LcFcRs contains a signal peptide and 2-8 Ig domains, and LcFcγRⅡ, LcFcγRⅢ and LcFcRL5 also contain a transmembrane region. Expression prolife analysis showed that LcFcγRⅠL, LcFcγRⅡ, LcFcγRⅡaL and LcFcγRⅢ were mainly expressed in mucous tissues such as gill or intestine, while LcFcRL5 and LcFcRLA were mainly expressed in immune tissues such as spleen and head kidney. Moreover, upon LPS the expression of these six FcRs genes in the spleen and head kidney was significantly up-regulated at different time points. After stimulation with poly (I:C) challenged, the six FcRs genes were significantly up-regulated in head kidney and spleen at different time points, whereas the expression of LcFcRL5 was significantly up-regulated in the spleen but significantly down-regulated in the head kidney. Results reveal that large yellow croaker FcRs may play different important roles in the immune response induced by LPS and poly (I:C). |
| Key words: marine biology large yellow croaker FcRs immunoglobulin molecular cloning |
