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抑制黄曲霉毒素的深海环状芽胞杆菌发酵条件优化
徐静静,闫培生
0
(哈尔滨工业大学环境学院,黑龙江 哈尔滨 150090;哈尔滨工业大学(威海)海洋科学与技术学院,山东 威海264209)
摘要:
环状芽胞杆菌(Bacillus circulans)菌株FA13在M2培养基中的发酵无细胞上清液对寄生曲霉(Aspergillus parasiticus)突变菌株NFRI-95菌丝生长和黄曲霉毒素的产生表现出明显的抑制作用。为了最大限度地提高活性物质的产量,本研究对从南大西洋深海沉积物中分离到的环状芽胞杆菌菌株FA13的发酵条件进行了均匀设计发酵优化。优化后,菌株FA13的发酵液10倍稀释液对寄生曲霉突变菌株NFRI-95的菌丝生长抑制率从10.98%提高到70.57%,黄曲霉毒素产生抑制率从41.43%提高到100.00%;对应的发酵条件为发酵温度38.70 ℃,接种量6.00%,海水体积占比0.00%,培养基pH 8.71,培养时间6 d。
关键词:  海洋生物学  环状芽胞杆菌  抑制产毒  寄生曲霉突变菌株  黄曲霉毒素  发酵工艺优化
DOI:10.3969/J.ISSN.2095-4972.2022.04.015
基金项目:国际海域资源调查与开发“十二五”重大项目资助项目(DY125-15-R-01)
Optimizing fermemtation conditions of a deep sea bacterium Bacillus circulans for inhibiting aflatoxin activity
XU Jingjing,YAN Peisheng
(School of Environment, Harbin Institute of Technology, Harbin 150090, China;School of Marine Science and Technology, Harbin Institute of Technology, Weihai, Weihai 264209, China)
Abstract:
Through liquid fermentation with M2 medium, the cell-free supernatant of Bacillus circulans FA13 strain, which isolated from deep-sea sediments of the South Atlantic Ocean, showed prominent inhibition activities against mycelial growth of Aspergillus parasiticus mutant strain NFRI-95 and aflatoxin producing. In order to maximize the yield of active substances, an uniform design was used to optimize fermentation conditions of the strain. After optimization, the inhibition efficacy of the 10-time diluted supernatant against Aspergillus parasiticus NFRI-95 mycelial growth and aflatoxin production was increased from 10.98% and 41.43% to 70.57% and 100.00%, respectively. The corresponding fermentation conditions were found as fermentation temperature 38.70 ℃, inoculum size 6.00%, volume fraction of seawater 0.00%, medium pH 8.71 and incubation time 6 days.
Key words:  marine biology  Bacillus circulans  inhibiting aflatoxin systhesis  Aspergillus parasiticus mutant strain  aflatoxin  fermentation process optimization

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