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基于线粒体控制区的中国东南沿海黄鳍棘鲷群体遗传多样性和遗传分化
叶幼玲,黄张帆,徐安乐,汪睛,黎中宝
0
(集美大学水产学院,福建 厦门 361021;福建省海洋渔业资源与生态环境重点实验室,福建 厦门 361021;福建省花鲈育种重点实验室,福建 福鼎355200)
摘要:
本研究通过对线粒体控制区序列进行分析的技术手段,对中国宁德(ND)、厦门(XM)、漳浦(ZP)、南澳岛(NAD)、湛江(ZJ)、海口(HK)与北海(BH)的7个野生黄鳍棘鲷(Acanthopagrus latus)群体的遗传多样性和遗传分化进行比较分析。结果表明:中国东南沿海的野生黄鳍棘鲷群体呈现出中等以上的遗传多样性特征,48个黄鳍棘鲷样品的控制区序列长度为580 bp,单倍型数目为44个,总体的单倍型多样性指数(Hd)和核苷酸多样性指数(Pi)分别为0.996和0.017 30。7个野生黄鳍棘鲷群体间的遗传分化程度较低,各群体间的遗传距离为0.010 28~0.029 87。群体间的遗传分化系数(FST)为-0.068 41~0.545 86,北海群体和其他群体间存在一定程度的遗传分化。AMOVA分析显示群体内的遗传变异占比为83.34%,大部分遗传变异发生在组群内;但北海群体与其他6个群体分为两个组群后,组群间的遗传变异占比为42.66%,两个组群间存在较大程度的遗传变异。基于Kimrua距离法构建的系统进化树中显示北海群体聚为一个分支,另外6个群体聚为一支。基于TCS network构建的单倍型网络图中44个单倍型混杂在一起,未按照水系格局和地理距离进行分布,表明黄鳍棘鲷群体间未形成多个单系群。本研究可为中国东南沿海野生黄鳍棘鲷群体的种质资源利用和保护提供科学依据。
关键词:  海洋生物学  黄鳍棘鲷  线粒体控制区  遗传多样性  遗传分化
DOI:10.3969/J.ISSN.2095-4972.2022.04.003
基金项目:福建省自然科学基金资助项目(2017J01638);福建省科技厅引导性项目资助项目(2020N0013)
Genetic diversity and genetic structure of Acanthopagrus latus populations in the southeastern coast of China based on the mitochondrial DNA control region analysis
YE Youling,HUANG Zhangfan,XU Anle,WANG Jing,LI Zhongbao
(Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Ecoenvironment, Xiamen 361021, China;Fujian Provincial Key Laboratory of Breeding on Lateolabrax Japonicus, Fuding 355200, China)
Abstract:
This study was conducted to analyze the genetic diversity and genetic structure of wild Acanthopagrus latus populations in the southeastern coast of China by mitochondrial DNA control region including Ningde, Xiamen and Zhangpu in Fujian Province, Nan'ao Island and Zhanjiang in Guangdong Province, Haikou in Hainan Province and Beihai in Guangxi Zhuang Autonomous Region. Results showed that the mitochondrial DNA control region sequence of 48 individuals is 580 bp, and the haplotypes were 44. The haplotype diversity and nucleotide diversity of populations were 0.996 and 0.017 30, respectively. The level of genetic differentiation among seven A. latus populations was low, genetic distance between each population was 0.010 28-0.029 87. The FST between populations was from -0.068 41 to 0.545 86, and there was a certain level of genetic differentiation between Beihai population and other populations. AMOVA analysis showed that the genetic variation within the population was 83.34%, and most of the genetic variations occurred within the population. When Beihai population and other six populations were divided into two groups, AMOVA analysis showed that the proportion of genetic variations between two groups was 42.66%, and the level of genetic variations between two groups was relatively high. The phylogenetic tree showed that Beihai population clustered into one branch, and the other six groups clustered into another branch. The haplotype network based on TCS network showed that 44 haplotypes were mixed together, and there were no monophyletic groups among the populations. In summary, this study potentially provided theoretical basis for fishery management and germplasm conservation in the future.
Key words:  marine biology  Acanthopagrus latus  mitochondrial DNA control region  genetic diversity  genetic differentiation

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