| 摘要: |
| “共生”和“钙化” 一直是珊瑚生物生态学研究中两个最核心的问题，为加深对珊瑚共生和钙化生物过程的理解，以丛生盔形珊瑚(Galaxea fascicularis)作为研究模型，通过透射电镜和扫描电镜研究珊瑚共生体的细胞显微结构及骨骼的形态特征，并利用电感耦合等离子体质谱(Inductively Coupled Plasma Mass Spectrometry, ICP-MS)对骨骼的微量元素进行成分分析。结果表明，珊瑚共生体的外膜层和内膜层分别起源于宿主细胞和虫黄藻细胞，珊瑚共生体膜的复杂结构对“珊瑚虫黄藻”的代谢物交换和相互作用具有重要意义。珊瑚外皮细胞可分泌大量的黏液而且体壁表面具有一定的流动性，可能与共生虫黄藻和微生物的穿梭有直接的关系。另外，珊瑚的骨骼显微结构表明，骨骼有机基质 (Skeletal Organic Matrix， SOM) 的分泌对于钙化晶体和骨架纤维的形成至关重要，支持珊瑚矿化由生物控制的学说。本研究加深了对共生珊瑚形态学的认识，为未来珊瑚应对海洋环境变化的细胞应激反应的研究奠定了基础。 |
| 关键词: 海洋生物学 珊瑚 钙化 共生 形态 |
| DOI：10.3969/J.ISSN.2095-4972.2022.01.005 |
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| 基金项目:国家自然科学基金资助项目（41906094）；福建省科技计划资助项目（2020N5011）；福州市“十三五”海洋经济创新发展示范资助项目（FZHJ11）；闽江学院科研专项资助项目（YSZ20016） |
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| Morphology of coral symbiosome and skeleton of Galaxea fascicularis |
| LIN Zhenyue,CHEN Jianming |
| (Institute of Oceanography, Minjiang University, Fuzhou 350108, China; Fuzhou Marine Ecological Aquaculture Industry Technology Innovation Center, Minjiang University, Fuzhou 350108, China) |
| Abstract: |
| Symbiosis and calcification are the most important issues in the coral ecology. To understand more biological process of coral symbiosis and calcification the coral Galaxea fascicularis was used as a model on which the coral-morphological characteristics of symbiont cells and skeletons were studied with transmission electron microscope (TEM) and scanning electron micrograph (SEM). It showed that the outer membrane layer and the inner membrane layer of the coral symbiont were originated from the host cells and the zooxanthellae cells, respectively. The complex structures of the coral symbiotic membranes played roles in the metabolite exchange and interaction in the couple of coralzooxanthellae. Coral skin cells could secrete a large amount of mucus and had a certain fluidity on the coral surface, which might be related to a shuttle dynamic of symbiotic zooxanthellae or microorganisms. In addition, the coral skeletal microstructures indicated that the secretion of organic matrix (SOM) was critical for the formation of the calcified crystals and skeletal fibers, supporting the viewpoint that coral mineralization is a biological regulation process. This study provides us further understanding of the coral morphology for the study of cellular stress responding to the marine environmental changes. |
| Key words: marine biology coral calcification symbiosis morphology |
