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海洋细菌Bacillus sp. Cs-700耐受及移除铯能力研究
张飞,郭亚平,于涛,纪建达,汤坤贤,吴元菲
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(自然资源部第三海洋研究所,福建 厦门 361005;福建省海洋生态保护与修复重点实验室, 福建 厦门 361005;南京大学地理与海洋科学学院,江苏 南京 210023;厦门大学化学化工学院,福建 厦门361005)
摘要:
为了筛选可以耐受高浓度铯离子(Cs+)的海洋细菌并探究细菌耐受Cs+的机制,本研究从广西近海沉积物样品中筛选出一株能够耐受700 mmol/dm3 Cs+的海洋细菌,是目前已报道的可耐受最高浓度Cs+的细菌。通过形态学分析、16S rRNA序列分析及生理生化特性的测定,确定该菌属于芽孢杆菌属(Bacillus),故将该菌株命名为Bacillus sp. Cs-700。在添加有700 mmol/dm3 氯化铯的培养基中,菌体对铯的移除率最高可达52.06%。研究结果表明Bacillus sp. Cs-700不但具有耐受高浓度Cs+ 的特性,还具有较好的铯移除能力,具有修复放射性铯污染环境的潜力。本研究可为探索海洋放射性铯的生态修复提供参考。
关键词:  海洋生物学  海洋细菌  铯离子  耐受性  移除能力
DOI:10.3969/J.ISSN.2095-4972.2022.01.002
基金项目:广西重点研发计划资助项目(2017AB30024);自然资源部第三海洋研究所基本科研业务费资助项目(海三科 2020013,海三科2018007);厦门海洋研究开发院共建资助项目(K200701)
Cesium tolerance and removal ability of marine bacterium Bacillus sp. Cs-700
ZHANG Fei,GUO Yaping,YU Tao,JI Jianda,TANG Kunxian,WU Yuanfei
(Third Institute of Oceanography, MNR, Xiamen 361005, China;Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration, Xiamen 361005, China;Third Institute of Oceanography, MNR, Xiamen 361005, China;School of Geography and Oceanography, Nanjing University, Nanjing 210023, China;College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China)
Abstract:
To screen marine bacteria which can tolerate a high concentration of cerium ion (Cs+) and its mechanism, we obtained a bacterium from the sediments of offshore area of Guangxi. The strain could survive in an environment of 700 mmol/dm3+ and is the bacterium that has been reported to tolerate the highest concentration of Cs+ . This bacterium was named Bacillus sp. Cs-700 after it had been identified by morphology, 16S rRNA sequence, and physiological and biochemical characteristics. In the medium supplemented with 700 mmol/dm3Bacillus sp. Cs-700 not only was able to tolerate high concentration of Cs+ but also had a good Cs+ removal ability, and had the potential to restore the environment contaminated by radioactive cesium. This study can be taken as reference for ecological restorations of environment contaminated by radioactive cesium.
Key words:  marine biology  marine bacterium  cesium ion  tolerance  removal ability

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