摘要: |
为了筛选可以耐受高浓度铯离子(Cs+)的海洋细菌并探究细菌耐受Cs+的机制,本研究从广西近海沉积物样品中筛选出一株能够耐受700 mmol/dm3 Cs+的海洋细菌,是目前已报道的可耐受最高浓度Cs+的细菌。通过形态学分析、16S rRNA序列分析及生理生化特性的测定,确定该菌属于芽孢杆菌属(Bacillus),故将该菌株命名为Bacillus sp. Cs-700。在添加有700 mmol/dm3 氯化铯的培养基中,菌体对铯的移除率最高可达52.06%。研究结果表明Bacillus sp. Cs-700不但具有耐受高浓度Cs+ 的特性,还具有较好的铯移除能力,具有修复放射性铯污染环境的潜力。本研究可为探索海洋放射性铯的生态修复提供参考。 |
关键词: 海洋生物学 海洋细菌 铯离子 耐受性 移除能力 |
DOI:10.3969/J.ISSN.2095-4972.2022.01.002 |
|
基金项目:广西重点研发计划资助项目(2017AB30024);自然资源部第三海洋研究所基本科研业务费资助项目(海三科 2020013,海三科2018007);厦门海洋研究开发院共建资助项目(K200701) |
|
Cesium tolerance and removal ability of marine bacterium Bacillus sp. Cs-700 |
ZHANG Fei,GUO Yaping,YU Tao,JI Jianda,TANG Kunxian,WU Yuanfei |
(Third Institute of Oceanography, MNR, Xiamen 361005, China;Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration, Xiamen 361005, China;Third Institute of Oceanography, MNR, Xiamen 361005, China;School of Geography and Oceanography, Nanjing University, Nanjing 210023, China;College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China) |
Abstract: |
To screen marine bacteria which can tolerate a high concentration of cerium ion (Cs+) and its mechanism, we obtained a bacterium from the sediments of offshore area of Guangxi. The strain could survive in an environment of 700 mmol/dm3+ and is the bacterium that has been reported to tolerate the highest concentration of Cs+ . This bacterium was named Bacillus sp. Cs-700 after it had been identified by morphology, 16S rRNA sequence, and physiological and biochemical characteristics. In the medium supplemented with 700 mmol/dm3Bacillus sp. Cs-700 not only was able to tolerate high concentration of Cs+ but also had a good Cs+ removal ability, and had the potential to restore the environment contaminated by radioactive cesium. This study can be taken as reference for ecological restorations of environment contaminated by radioactive cesium. |
Key words: marine biology marine bacterium cesium ion tolerance removal ability |