| 摘要: |
| 太平洋牡蛎（Crassostrea angulata）是中国主要的海洋养殖经济物种之一，具有重要的经济价值和商业价值，附着变态是牡蛎幼体从受精卵发育到成体所需的重要阶段，其分子机制的研究对牡蛎养殖育苗具有重要的指导意义.本研究利用双向电泳技术分离筛选牡蛎幼体附着变态过程中的差异表达的蛋白，进一步利用质谱技术鉴定幼体附着变态过程中发育相关的关键蛋白，共筛选出128个差异蛋白，鉴定并注释了39种蛋白.通过对差异表达蛋白功能分析，筛选获得了与纤毛组建相关的筑丝蛋白和与稳定钙离子胞内浓度相关的钙网蛋白，为揭示牡蛎幼体面盘组织及纤毛的退化消失和钙离子有效诱导幼体附着变态的机制提供分子数据.同时还获得了调节脂肪酸β-氧化、三羧酸循环和糖酵解重要生理功能的关键酶蛋白：乙酰辅酶A脱氢酶、异柠檬酸脱氢酶和3磷酸甘油醛脱氢酶，以及调节无脊椎动物机体能量存储和释放的酶蛋白：精氨酸激酶和ATP合成酶，进一步阐明了牡蛎幼体在附着变态过程中能量调节的分子机制. |
| 关键词: 海洋生物学；牡蛎 幼体 附着变态 蛋白质组 |
| DOI：10.3969/J.ISSN.2095-4972.2018.03.009 |
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| 基金项目:福建省教育厅福建省中青年教师教育科研资助项目（JA15811）；厦门市科技局科技计划资助项目（3502Z20154078）；厦门医学院自然科学类科研计划资助项目（Z2014-08） |
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| Study on differentially expressed proteins related to larval settlement and metamorphosis in the Pacific oyster, Crassostrea gigas |
| YANG Bing-ye,PU Fei,CHEN Zhong-wei |
| (Xiamen Key Laboratory of Marine Medicinal Natural Products and Cell Engineering, Xiamen Medical College, Xiamen 361023, China; College of Ocean and Earth Science, Xiamen University, Xiamen 361102, China) |
| Abstract: |
| The Pacific oyster, Crassostrea gigas, is an economically important mariculture species in China, settlement and metamorphosis is an essential process for the larval development of the species. The study of molecular mechanism on larval settlement and metamorphosis is helpful for the oyster growing and seeding. In our studies 128 proteins in different expression were screened and 39 proteins identified during the settlement and metamorphosis with two-dimension gel electrophoresis and mass-spectrometric technique, respectively. The function analysis of the different expressed proteins screened and resulted fibroin related to the formation of cilia and the calcium net proteins related to stabilize the intracellular concentration of calcium ions. These provide the molecular data that reveals the degeneration and disappearance of the veliger tissue and cilia of the oyster larvae and the mechanism of calcium ion to effectively induce the attachment and metamorphosis of the larvae. In the meanwhile, we obtained three key protein enzymes as acetyl coenzyme A dehydrogenase, isocitrate dehydrogenase and 3-phosphate glyceraldehyde dehydrogenase, which respectively regulate the important physiological functions of fatty acid β-oxidation, tricarboxylic acid cycle and glycolysis, and two other enzymes, arginine kinase and ATP synthetase that regulate the energy storage and release in the invertebrate. These observations furtherly clarify the molecular mechanism of energy regulation in oyster larvae during the settlement and metamorphosis. |
| Key words: marine biology oyster larvae settlement and metamorphosis proteome |
