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| 2株海马肠道益生菌的筛选与初步鉴定 |
| 周治东,张跃平,骆巧琦,李海平,连张飞,张丽艳,郭炳坚,林荣光,李纪忠 |
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| (福建海洋研究所，福建 厦门 361013;福建省海陆界面生态环境联合重点实验室，福建 厦门 361005;福建省海岛与海岸带管理技术研究重点实验室，福建 厦门 361013;福建港德水产有限公司，福建 福州 350000) |
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| 摘要: |
| 从正常养殖的健康线纹海马（Hippocampus erectus）肠道中分离纯化到52株细菌，通过溶血性实验对肠道菌群进行初筛，结果发现52株肠道细菌均不产生溶血素，不具有潜在的致病性.以纸片扩散法在胞外蛋白酶选择培养基上对菌株产蛋白酶的能力进行测试，筛选出2种能够分泌胞外蛋白酶的菌株.动物安全性实验证实2株实验菌对海马无明显的毒害作用.海马生长参数测定实验显示投喂菌株的实验组体长、体重的日平均增长率均高于不投喂菌株的对照组，证明所筛选菌株具有一定的生长促进作用，有望对其进行海马养殖微生物制剂的开发.随后测定了2株细菌的16S rRNA 基因序列，分析相应细菌序列的同源性，构建系统发育树，结合菌株生理生化特性及其表型特征，分别将Hm7与Hm28鉴定为Bacillus horikoshii和Pseudoalteromonas carrageenovora. |
| 关键词: 海洋生物学 海马 肠道益生菌 蛋白酶 水产养殖 菌种鉴定 |
| DOI：10.3969/J.ISSN.2095-4972.2018.02.011 |
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| 基金项目:福建省公益类科研院所专项资助项目（2014R1006-7） ；福建省海洋经济创新发展区域示范资助项目（2014FJ-16）； “十三五”厦门市海洋经济创新发展示范资助项目(16PZY002SF-18) |
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| Screening and identification of two probiotics strains from sea horse intestine |
| ZHOU Zhi-dong,ZHANG Yue-ping,LUO Qiao-qi,LI Hai-ping,LIAN Zhang-fei,ZHANG Li-yan,GUO Bing-jian,LIN Rong-guang,LI Ji-zhong |
| (Fujian Institute of Oceanology， Xiamen 361013， China; Fujian Provincial Key Laboratory of Coastal Ecology and
Environmental Studies， Xiamen 361005， China;Fujian Provincial Key Laboratory of Coast and Island Management
Technology Study， Xiamen 361013， China;Fujian Gang De Aquatic Products Limited Company， Fuzhou 350000， China) |
| Abstract: |
| A total of 52 bacterium strains were isolated from healthy sea horse （Hippocampus erectus）. The hemolysis test was carried out to show that all the bacteria did not product hemolysin， so they were not pathogenic. The ability of producing protease was tested by the method of disk diffusion on the extracellular proteinase selective medium， and 2 strains secreting extracellular protease had been screened out. Safety experiments showed that 2 strains of experimental bacteria were not toxic to sea horse. The growth parameters experiment showed that the daily growth rate of body length and body weight from experimental groups were higher than the control group that fed no probiotics. Its proved that the screening strains have certain growth promoting effect， were expected to be used on aquaculture microbial preparation. Then the 16S rRNA gene sequences of 2 strains were determined. The homology of related strain sequences was analyzed for the phylogenetic tree. Combined with the biochemical phenotype and physiological characteristics， Hm7 and Hm28 were identified as Bacillus horikoshii and Pseudoalteromonas carrageenovora． |
| Key words: marine biology sea horse intestinal probiotics protease aquaculture strain identification |