| 摘要: |
| 激活转录因子-2（ATF2）作为细胞内应激活化蛋白激酶下游效应分子，参与调控了生物体诸如细胞增殖、凋亡以及免疫炎症反应等许多细胞生物学过程.本研究首次从凡纳滨对虾（Litopenaeus vannamei）中克隆得到了ATF-2基因全长编码序列，命名为LvATF-2.序列分析表明，LvATF-2基因开放阅读框长1 719 bp，可编码572个氨基酸.其编码的蛋白具有特征性的锌指结构和碱性亮氨酸拉链结构域，并且在N端具有两个保守的苏氨酸磷酸化位点（Thr71和Thr73）.进一步的系统进化树分析显示，LvATF-2蛋白与脊椎动物及软体动物ATF-2蛋白聚为一簇，且与软体动物ATF-2蛋白亲缘关系更近.半定量RT-PCR结果显示，LvATF-2基因在所检测的各个组织中均有转录，但在鳃和肠道中转录量较高.此外LvATF-2基因在白斑综合症病毒（WSSV） 感染早期转录水平显著下降，提示该基因与WSSV感染密切相关，可能参与了对虾应答病毒的免疫反应过程.本文通对LvATF-2基因的克隆与表达特征分析，为将来研究该基因在对虾免疫应答过程中的功能提供了依据. |
| 关键词: 海洋生物学 凡纳滨对虾 激活转录因子-2（ATF-2） 白斑综合症病毒（WSSV） 分子克隆 |
| DOI：10.3969/J.ISSN.2095-4972.2016.02.006 |
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| 基金项目:现代农业产业技术体系专项资金资助项目(CARS-47)；国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(2014020) |
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| Molecular cloning and expression analysis of ATF2 from the shrimp Litopenaeus vannamei |
| YAO Defu,RUAN Lingwei,SHI Hong |
| (School of Life Science，Xiamen University，Xiamen 361005，China;Key Laboratory of Marine Genetic Resources of State Oceanic Administration，Third Institute of Oceanography，SOA，Xiamen 361005，China) |
| Abstract: |
| ATF-2，as a downstream effector of cellular stress-activated protein kinases，participates in the regulation of numerous cell processes，including cell proliferation and differentiation，cell cycle，apoptosis and immune inflammatory response. In the present study，a ATF-2 gene from Litopenaeus vannmei was cloned for the first time and designated as LvATF-2. Sequence analysis showed that the open reading frame of LvATF-2 gene was 1 719 bp in length and encoded a protein with 572 amino acids. The LvATF-2 protein contained characteristic zinc finger and bZIP domains，and two conserved threonine phosphorylation sites （Thr71 and Thr73） at the N-terminus. Phylogenetic tree analysis further displayed that LvATF-2 protein was clustered with vertebrate and mollusc ATF-2 protein and showed a closer relationship with ATF-2 protein of the mollusc. Semi-quantitative RT-PCR showed that LvATF-2 mRNAs were transcribed in all examined tissues，but the mRNA level of LvATF-2 gene was relatively higher in intestine and gill tissues. In addition，the transcriptional level of LvATF-2 gene was remarkably down-regulated during the early stage of WSSV infection，suggesting that LvATF-2 gene was closely related to WSSV infection and might be involved in immune response upon viral infection. Our study on LvATF-2 gene might have provided useful clue for exploring its function in shrimp immune response in future. |
| Key words: marine biology v Litopenaeus vannamei activating transcription factor-2 （ATF-2） white spot syndrome virus （WSSV） molecule cloning |
