| 摘要: |
| 对4种假单胞菌产脓菌素培养基进行筛选，确定琥珀酸钠培养基为基础培养基，并考察了添加不同物质对假单胞菌脓菌素产量的影响，结果表明，向琥珀酸钠培养基中添加甘油、海盐、硝酸铵时对铁载体产量有明显的促进作用.采用正交实验法获得了高产脓菌素的最佳发酵培养基配方：4 g/dm3琥珀酸钠，6 g/dm3 K2HPO4，3 g/dm3 KH2PO4，1 g/dm3 （NH4）2SO4，1 g/dm3 NH4NO3，5 g/dm3海盐，1.5%甘油.其脓菌素产量最高可达到265 mg/dm3，约是基础培养基产量的7倍以上，此培养基成分简单不含复杂的有机氮源，脓菌素产量高，有利于脓菌素的进一步分离纯化与制备.同时本实验对其发酵条件进行优化研究，结果确定最佳发酵条件：培养基起始pH值为6.9，接种量 1.0%，培养温度为25℃，转速为220 r/min，发酵时间为28 h. |
| 关键词: 海洋生物学 假单胞菌 脓菌素 培养基优化 发酵条件 |
| DOI：10.3969/J.ISSN.2095-4972.2016.02.003 |
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| 基金项目:国家海洋局海洋公益性行业科研专项经费资助项目(201205020) |
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| Screening of original medium and optimizing fermentation conditions of marine derived Pseudomonas fluorescens for high-producing pyoverdines |
| SHANG Weiwei,HOU Luhong |
| (Key Laboratory of Marine Biogenetic Resources，Third Institute of Oceanography，SOA，Xiamen，361005，China) |
| Abstract: |
| Base medium of Pseudomonas was screened for producting pyoverdines among four kinds of media， it was showed that sodium succinate medium as basic medium and the effects of additions of different components on the output of pyoverdines were examined. The results showed that the production of pyoverdines could be promoted significantly， while glycerin， sea salt and NH4NO3 added in sodium succinate medium. Sodium succinate comound medium was screened and optimized by the orthogonal experiment: 1.5% glycerol， 4 g/dm3sodium succinate， 6 g/dm3 K2HPO4， 3 g/dm3 KH2PO4， 1 g/dm3 （NH4）2SO4， 1 g/dm3 NH4NO3， 5 g/dm3 sea salt. The highest production of pyoverdines is 265 mg/dm3 and more than 7 times than before. The medium is simple and advantageous to isolate and purify the pyoverdines.At the same time， the fermentation conditions were optimized and the best conditions were determined. The results showed that initial pH， inoculation amount， temperature， shaking speed and the time were 6.9，1.0%，25℃，220 r/min and 28h， respectively． |
| Key words: marine biology Pseudomonas sp. pyoverdines medium optimization fermentation condition |
