| 摘要: |
| 为研究DNA条形码技术在厦门海域鱼类分类鉴定中的可行性，随机选取了厦门海域23种鱼类样品进行COⅠ基因扩增，结果共获取23条序列，平均长度为656 bp，序列中T、C、G、A碱基的平均含量分别为：29.40%、28.10%、18.40%、24.10%.样品种内、种间、科内和目内的遗传距离（K2P）分别为：0.21%， 20.50%、24.47% 和 25.62%，遗传距离随着分类阶元的提高而增大，种间遗传距离明显大于种内遗传距离.所选厦门海域鱼类样品仅蓝圆鯵鉴定到圆鯵属，未能鉴定到种，其余22种全部鉴定到种，表明COⅠ基因序列可以作为鱼类分类鉴定的有效DNA条形码. |
| 关键词: 海洋生物学 鱼类 细胞色素C氧化酶亚基Ⅰ基因 DNA条形码 物种鉴定 厦门海域 |
| DOI：10.3969/J.ISSN.2095-4972.2016.01.018 |
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| 基金项目:福建省科技厅计划资助项目（2013N0021）；国家海洋局第三海洋研究所基本科研经费资助项目（海三科2013001） |
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| Species identification for fishes in Xiamen sea waters based on COⅠ barcoding |
| XING Bing peng,LIN Ru rong,WANG Yan guo,ZHANG Zhi lan |
| (Third Institute of Oceanography，SOA，Xiamen 361005，China) |
| Abstract: |
| In this study， we collected DNA barcodes based on cytochrome oxidase subunit Ⅰ（COⅠ） sequences from 23 species of fish specimens captured in Xiamen sea waters. The mean length of the sequences was 656 bp and the average content of T， C， G and A were 29.4%， 28.1%， 18.4% and 24.1%， respectively. The average Kimura two parameter （K2P） distances within-species， genus， family and order were 0.21%， 20.50%， 24.47% and 25.62%， respectively. Interspecific genetic distance was much larger than intraspecific distance from composition. K2P genetic distance values were found increasing with taxonomic level. All species， except for Decapterus maruadsi， can be differentiated by their COⅠ sequence. High efficiency of species identification was demonstrated in the present study by DNA barcoding， and we conclude that COⅠ sequencing can be used to identify fish species． |
| Key words: marine biology fishes cytochrome oxidase subunit Ⅰ（COⅠ） DNA barcoding species identification Xiamen sea waters |
